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guinea pig anti tlx3  (R&D Systems)


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    R&D Systems guinea pig anti tlx3
    Guinea Pig Anti Tlx3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/guinea+pig+anti+tlx3/Human+TrkA+Antibody/10__1523_slash_jneurosci__1441___23__2024-72-34-49
    Average 93 stars, based on 23 article reviews
    guinea pig anti tlx3 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Molecular Characterization of Nodose Ganglia Development Reveals a Novel Population of Phox2b+ Glial Progenitors in Mice
    Article Snippet: .. Immunofluorescence experiments were performed on cryosections using the following primary antibodies: rat anti-GFP (Nacalai Tesque, GF090R, 1:1,000), goat anti-Phox2b (R&D Systems, AF4940, 1:200), rabbit anti-Sox10 (Abcam, ab227680, 1:200), goat anti-Sox10 (R&D Systems, AF2864, 1:200), guinea pig anti-Tlx3 (1:5,000, kindly provided by Thomas Müller; Müller et al., 2005), goat anti-TrkA (R&D Systems, AF175, 1:500), goat anti-TrkB (R&D Systems, AF1494, 1:300), chicken anti-βgal (Abcam, ab134435, 1:1,000), chicken anti-GFP (Aves Labs, GFP-1020, 1:500), rabbit anti-RFP (Rockland Immunochemicals, 600-401-379-RTU, 1:500), goat anti-RFP (Biorbyt, orb334992, 1:500), and rabbit anti-Phox2b (1:500, kindly provided by Jean-François Brunet). ..

    Article Title: Molecular characterization of nodose ganglia development reveals a novel population of Phox2b+ glial progenitors in mice
    Article Snippet: .. Immunofluorescence experiments were performed on cryosections using the following primary antibodies: rat anti-GFP (Nacalai Tesque, GF090R, 1:1000), goat anti-Phox2b (R&D Systems, AF4940, 1:200), rabbit anti-Sox10 (Abcam, ab227680, 1:200), goat anti-Sox10 (R&D Systems, AF2864, 1:200), guinea pig anti-Tlx3 (1:5000, kindly provided by Thomas Müller), goat anti-TrkA (R&D Systems, AF175, 1:500), goat anti-TrkB (R&D Systems, AF1494, 1:300), chicken anti-βgal (Abcam, ab134435, 1:1000), chicken anti-GFP (Aves Labs, GFP-1020, 1:500), rabbit anti-RFP (Rockland, 600-401-379-RTU, 1:500), goat anti-RFP (Biorbyt, orb334992, 1:500), and rabbit anti-Phox2b (1:500, kindly provided by Jean-François Brunet). ..



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    ( A ) GFP (green) and PAX2 (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification showing contacts between Pax2 and 5-HT fibers. Scale bar, 50 μm. ( B ) GFP (green) and <t>Tlx3</t> (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification, low or no contact. Scale bar, 25 μm. ( C ) After 3D reconstruction (see Materials and Methods) quantification of contacts between cell bodies (Pax2 and Tlx3) and 5-HT fibers. ( C ) Contacted cells are significantly higher for Pax2(+) than for Tlx3(+) in both males and females [males (blue): 86.2 ± 4.6% for Pax2 and 15.5 ± 3.8% for Tlx3, P < 0.001, Mann-Whitney; females (red): 76.3 ± 2.9% for Pax2 and 8.3 ± 2.2% for Tlx3, P < 0.001, Mann-Whitney]. ( D ) Immunostaining against TPH2 in slices of lumbar spinal cord of GAD67::GFP mice. Scale bar, 25 μm. GABA neurons [GFP(+) in green, stars] or fibers in DH are contacted by TPH2-positive putative synaptic button (in red, white arrows). ( E ) Expression of Myc-tag in synaptic 5-HT terminals in DH following injections of an AAV-synaptophysin-Myc. Immunoreactivity is present in the deeper lamina of the DHSC. Scale bar, 100 μm. Higher magnification shows 5-HT axonic button (white arrow). Scale bar, 20 μm. ( F ) Myc(+) synaptic buttons are in direct contact with GAD67-positive cells and fibers in epet::cre/ GAD67::GFP mice. Scale bar, 20 μm. Inset (top): Myc(+) button on GAD(+) cell bodies. Inset (bottom): Myc(+) button on GAD(+) on GAD(+) fibers.
    Guinea Pig Anti Tlx3 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/guinea+pig+anti+tlx3/TLX3+Antibody/pmc09328683-298-26-40
    Average 92 stars, based on 1 article reviews
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    Image Search Results


    ( A ) GFP (green) and PAX2 (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification showing contacts between Pax2 and 5-HT fibers. Scale bar, 50 μm. ( B ) GFP (green) and Tlx3 (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification, low or no contact. Scale bar, 25 μm. ( C ) After 3D reconstruction (see Materials and Methods) quantification of contacts between cell bodies (Pax2 and Tlx3) and 5-HT fibers. ( C ) Contacted cells are significantly higher for Pax2(+) than for Tlx3(+) in both males and females [males (blue): 86.2 ± 4.6% for Pax2 and 15.5 ± 3.8% for Tlx3, P < 0.001, Mann-Whitney; females (red): 76.3 ± 2.9% for Pax2 and 8.3 ± 2.2% for Tlx3, P < 0.001, Mann-Whitney]. ( D ) Immunostaining against TPH2 in slices of lumbar spinal cord of GAD67::GFP mice. Scale bar, 25 μm. GABA neurons [GFP(+) in green, stars] or fibers in DH are contacted by TPH2-positive putative synaptic button (in red, white arrows). ( E ) Expression of Myc-tag in synaptic 5-HT terminals in DH following injections of an AAV-synaptophysin-Myc. Immunoreactivity is present in the deeper lamina of the DHSC. Scale bar, 100 μm. Higher magnification shows 5-HT axonic button (white arrow). Scale bar, 20 μm. ( F ) Myc(+) synaptic buttons are in direct contact with GAD67-positive cells and fibers in epet::cre/ GAD67::GFP mice. Scale bar, 20 μm. Inset (top): Myc(+) button on GAD(+) cell bodies. Inset (bottom): Myc(+) button on GAD(+) on GAD(+) fibers.

    Journal: Science Advances

    Article Title: Switch of serotonergic descending inhibition into facilitation by a spinal chloride imbalance in neuropathic pain

    doi: 10.1126/sciadv.abo0689

    Figure Lengend Snippet: ( A ) GFP (green) and PAX2 (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification showing contacts between Pax2 and 5-HT fibers. Scale bar, 50 μm. ( B ) GFP (green) and Tlx3 (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification, low or no contact. Scale bar, 25 μm. ( C ) After 3D reconstruction (see Materials and Methods) quantification of contacts between cell bodies (Pax2 and Tlx3) and 5-HT fibers. ( C ) Contacted cells are significantly higher for Pax2(+) than for Tlx3(+) in both males and females [males (blue): 86.2 ± 4.6% for Pax2 and 15.5 ± 3.8% for Tlx3, P < 0.001, Mann-Whitney; females (red): 76.3 ± 2.9% for Pax2 and 8.3 ± 2.2% for Tlx3, P < 0.001, Mann-Whitney]. ( D ) Immunostaining against TPH2 in slices of lumbar spinal cord of GAD67::GFP mice. Scale bar, 25 μm. GABA neurons [GFP(+) in green, stars] or fibers in DH are contacted by TPH2-positive putative synaptic button (in red, white arrows). ( E ) Expression of Myc-tag in synaptic 5-HT terminals in DH following injections of an AAV-synaptophysin-Myc. Immunoreactivity is present in the deeper lamina of the DHSC. Scale bar, 100 μm. Higher magnification shows 5-HT axonic button (white arrow). Scale bar, 20 μm. ( F ) Myc(+) synaptic buttons are in direct contact with GAD67-positive cells and fibers in epet::cre/ GAD67::GFP mice. Scale bar, 20 μm. Inset (top): Myc(+) button on GAD(+) cell bodies. Inset (bottom): Myc(+) button on GAD(+) on GAD(+) fibers.

    Article Snippet: For molecular identifications of the spinal network, spinal cord sections were incubated free-floating in 0.1 M PBS containing Triton X-100 (0.3%), bovine serum albumin (1%; Sigma-Aldrich), guinea pig anti-TLX3 antibody (1:1000; a gift from T. Müller), goat anti-Pax2 antibody (1:300; Bio-techne), rabbit anti-Myc antibody (1:500; Euromedex), rabbit anti-TPH2 antibody (1:1000; Bio-techne), and rabbit anti-GAD65/67 (G5163, Sigma-Aldrich) in combination with chicken anti-GFP antibody (1:1000; Averlabs) overnight at 4°C.

    Techniques: Immunolabeling, MANN-WHITNEY, Immunostaining, Expressing